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( A ) Schematic illustration of the in vitro DO model; concentration of cisplatin, 2.7 μg/ml; <t>EV/GOE,</t> 1 × 10 6 particles/ml; time of treatment, 24 hours (h). ( B ) Flow cytometry analysis of GC viability, apoptosis, and cell death. ( C to E ) Quantification of flow cytometry data, showing the efficacy of EVs in treating DO in vitro. ( F ) No statistically significant difference in apoptosis rates between GOEs <t>and</t> <t>GDF-9</t> –overexpressing MSCs. n = 3, data are presented as means ± SD. This figure was created in part using BioRender.com . Created in BioRender. Zhang, S. (2025) https://BioRender.com/zv1tbzt . IC 50 , median inhibitory concentration. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant.
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( A ) Schematic illustration of the in vitro DO model; concentration of cisplatin, 2.7 μg/ml; EV/GOE, 1 × 10 6 particles/ml; time of treatment, 24 hours (h). ( B ) Flow cytometry analysis of GC viability, apoptosis, and cell death. ( C to E ) Quantification of flow cytometry data, showing the efficacy of EVs in treating DO in vitro. ( F ) No statistically significant difference in apoptosis rates between GOEs and GDF-9 –overexpressing MSCs. n = 3, data are presented as means ± SD. This figure was created in part using BioRender.com . Created in BioRender. Zhang, S. (2025) https://BioRender.com/zv1tbzt . IC 50 , median inhibitory concentration. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant.

Journal: Science Advances

Article Title: Repair of female reproductive function by GDF-9–overexpressing extracellular vesicles via ACVR1B/SMAD2 regulation in ovarian granulosa

doi: 10.1126/sciadv.adw9006

Figure Lengend Snippet: ( A ) Schematic illustration of the in vitro DO model; concentration of cisplatin, 2.7 μg/ml; EV/GOE, 1 × 10 6 particles/ml; time of treatment, 24 hours (h). ( B ) Flow cytometry analysis of GC viability, apoptosis, and cell death. ( C to E ) Quantification of flow cytometry data, showing the efficacy of EVs in treating DO in vitro. ( F ) No statistically significant difference in apoptosis rates between GOEs and GDF-9 –overexpressing MSCs. n = 3, data are presented as means ± SD. This figure was created in part using BioRender.com . Created in BioRender. Zhang, S. (2025) https://BioRender.com/zv1tbzt . IC 50 , median inhibitory concentration. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant.

Article Snippet: The protein concentration of GDF-9 in control EVs and GOE was quantitatively analyzed using the ELISA kit (Raybiotech, ELH-GDF9-1).

Techniques: In Vitro, Concentration Assay, Flow Cytometry